Immunofluorescence analysis for ERK and cardiac Troponin I (cTnI) in the border zones of heart sections 14 days post myocardial infarction, following daily injection of BMP7 or water as control (n= 8 mice; a total of 9,318 cardiomyocytes have been analyzed). Cardiomyocytes with nuclear ERK immunoreactivity were counted manually within border zones in tissue sections; every dot represents a different heart (biological replicate), which in turn has been calculated by the analysis of one to two sections. Representative pictures are provided; arrows point at ERK-positive cardiomyocytes; scale bars, 20 μm.
