Evaluation of the role of BMP receptors in BMP7-induced cardiomyocyte proliferation by immunofluorescence analysis of DNA synthesis (BrdU assay) in neonatal (postnatal day 1, P1) cardiomyocytes following BMPR1A, ACVR1, ACVR1B, ACVRL1, BMPR2, and ACVR2A knockdown, upon BMP7 stimulation at 10 ng/mL for 48 h (n= 23,895 cardiomyocytes pooled from the analysis of 83 samples); representative pictures are provided; scale bars, 20 m, arrows point at proliferating cardiomyocytes. The values in (A)–(E) are presented as mean (error bars show SEM); statistical significance was determined using one-way ANOVA followed by Sidak’s test (comparison between pairs of treatments) in (A), (B), (D), and (E); and using two-sided Student’s t test in (C).
