Box Plot from Scientific Research

Western blot analysis of phospho-SMAD5, SMAD5, and GAPDH protein levels in enriched neonatal (postnatal day 1, P1) cardiomyocytes, separated from stromal cells by immunomagnetic separation, following BMP7 stimulation at 100 ng/mL for 30, 60, and 120 min (n= 3 replicates per condition). GAPDH protein levels are provided as second loading control (see also Figures S10D). The values in (A)–(E) are presented as mean (error bars show SEM); statistical significance was determined using one-way ANOVA followed by Sidak’s test (comparison between pairs of treatments) in (A), (B), (D), and (E); and using two-sided Student’s t test in (C).

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