In vivo evaluation of adult cardiomyocyte proliferation by immunofluorescence analysis of KI67 and cardiac Troponin T (cTnT) in the border and remote zones of heart sections 14 days post myocardial infarction, following daily injection of BMP7 or water as control (n= 8 mice; for KI67/cTnT staining a total of 14,345 cardiomyocytes have been analyzed in the border zones and 23,085 cardiomyocytes in the remote zones). Each dot represents a different heart (biological replicate), which is the average of the analysis of two to three sections. Representative pictures show images taken by confocal microscopy showing the xy maximum intensity projection of the stacks acquired from the sample (the slices view on the bottom and left of each panel represent the xz and yz stacks, respectively); arrows point at proliferating cardiomyocytes; scale bars, 25 m.
