Immunofluorescence analysis of PCNA (proliferating cell nuclear antigen) and myosin light chain 7 (MYL7, to identify cardiomyocytes) in heat-shocked hsp70L:bmp7b,myl7:eGFPaf5Tg transgenic and wild-type siblings at 7 dpi. Note that GFP fluorescence is not shown. n(wild-type) = 12 hearts, two sections per heart, 421 cardiomyocytes. n(hsp70L:bmp7b,myl7:eGFP) = 9 hearts, two sections per heart, 472 cardiomyocytes. EdU- and PCNA-positive cardiomyocytes in (B) and (D), respectively, were counted manually within the border zone of control and transgenic fishes. Each dot represents a different heart (biological replicate), which is the average of the analysis of two to three sections. Representative pictures are provided; arrows point at cycling cardiomyocytes; scale bars, 50 m. The values in (B) and (D) are presented as mean, error bars show SEM, statistical significance was determined using two-sided Student’s t test.
