Immunoblotting analysis shows that PTEN+RPTOR double mutant rescues the hyperactivation of mTORC1 (pS6) and PTEN+RICTOR double mutant rescues the hyperactivation of mTORC2 (pAKT) in WIBR3 human NPs. Molecular weight markers are shown on the right (kDa). Each data point represents an independent experiment (different hPSC clone or independent differentiation, n= 4 for each group). p values are calculated using post hoc multiple comparison after ANOVA.
