Quantification show that both PTEN+RPTOR and PTEN+RICTOR double mutants normalize the overproliferation PTEN mutant WIBR3 human NPs, as measured by KI67 immunostaining and EdU incorporation assay. Scale bar, 50 m. Each data point represents an independent experiment (different hPSC clone or independent differentiation, n= 4 for each group). p values are calculated using post hoc multiple comparison after ANOVA. Results are mean SEM.p< 0.01,p< 0.001, andp< 0.0001. See alsoFigure S8.
