Immunoblotting analysis shows that chronic rapamycin treatment (100 nM) reduces the hyperactivation of both mTORC1 (pS6) and mTORC2 (pAKT) inPTENmutant WIBR3 human NPs. Molecular weight markers are shown on the right (kDa). Each data point represents an independent experiment (different hPSC clone or independent differentiation,n= 4 for each group).pvalues are calculated using post hoc multiple comparison after ANOVA.
