Multi-electrode array analysis shows that both PTEN+RPTOR and PTEN+RICTOR double-mutant human neurons rescue the electric hyperactivity (J) and hypersynchronization (K) phenotypes. Raster plots (I) show spike distribution on day 25. The purple rectangle highlights the incidence of network burst. ANOVA found significant effects of genotype (black asterisks marked on the right side of the graphs). Post hoc multiple comparison test revealed differences on individual days of recording, in which the black asterisk represents a comparison between control and PTEN mutant neurons, the red asterisk represents a comparison between PTEN and PTEN+RPTOR mutant neurons, and the green asterisk represents a comparison between PTEN and PTEN+RICTOR mutant neurons (n= 4 for each group from 4 independent experiments). Results are mean ± SEM.*p< 0.05,**p< 0.01,***p< 0.001, and****p< 0.0001. See alsoFigure S4–S7.
