Multi-electrode array analysis shows that chronic rapamycin treatment (20 nM) rescues the electric hyperactivity (F) and hypersynchronization (G) phenotypes of PTEN mutant neurons. Raster plots (E) show spike distribution on day 25. The purple rectangle highlights the incidence of network burst. ANOVA found significant effects of genotype and rapamycin treatment (black asterisks marked on the right side of the graphs). Post hoc multiple comparison test revealed differences on individual days of recording, in which the black asterisk represents a comparison between vehicle-treated control and PTEN mutant neurons, the green asterisk represents a comparison between rapamycin- and vehicle-treated control neurons, and the red asterisk represents a comparison between rapamycin- and vehicle-treated PTEN mutant neurons (n= 4 for each group from 4 independent experiments). Results are mean ± SEM.*p< 0.05,**p< 0.01,***p< 0.001,****p< 0.0001, and nsp> 0.1. See also Figure S3.
