Multi-electrode array analysis of control and PTEN mutant neurons showing spike distribution on day 25 (raster plot, J) and mean firing rate (K) and synchronicity (L) over the time course of 30 days. The purple rectangle highlights the incidence of network burst. ANOVA found significant effects of genotype (black asterisks marked on the right side of the graphs). Post hoc multiple comparison test revealed differences on individual days of recording, in which the black asterisk represents a comparison between control and PTEN mutant neurons. PTEN mutant neurons are hyperactive and hypersynchronized (n= 3 for each group from 3 independent hPSC clones). Results are mean ± SEM.∗∗p< 0.01,∗∗∗p< 0.001, and∗∗∗∗p< 0.0001. See also Figures S1 and S2.
