Immunoblotting analysis shows hyperactivation of both mTORC1 (pS6, E and F) and mTORC2 (pAKT, E and G) in PTEN mutant human neurons on day 10. Molecular weight markers are shown on the right (kDa). Each data point represents an independent experiment (different hPSC clone or independent differentiation, n= 5 for each group). p value is calculated using Student’s t tests.
