Microscopy from Scientific Research

Alizarin red and Alcian blue staining of bones of the lateral view of the cervical vertebrae (C1–C7) of wild-type and Tbx1-KO mice. In Tbx1-KO neonates, the cervical vertebrae are hypoplastic, the anterior arch of C1 (ar) is missing, the body of the hyoid bone (hb) is absent, and the thyroid cartilages (tc) are hypoplastic. Diagrams of the vertebrae with the landmarks for the measured parameters in (D) are also shown.

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