Effect ofE2F1overexpression on allele-specific enhancer activity of rs41298798 towardTBX1. The rs41298798-C or rs41298798-G alleles were cloned downstream ofTBX1-promoter-driven luciferase constructs, and luciferase reporter assays were performed following transient transfection of MG63 or COS1 cells (n =6; the results are presented as mean SEM;*p 0.05;***p 0.001;****p 0.0001; two-way analysis of variance [ANOVA]).
