Ratio of delaminated (I) and Brachyury+ (J) cells in spheroids from (H). Data are shown as mean ± SEM. Each dot represents a single spheroid. In (I) and (J), n = 37 (WT) and 32 (PorcnKO) spheroids. 3 independent experiments. Mann-Whitney U test.****p < 0.0001; ns, non-significant.