Ratio of differentiated cells in 3D EpiSCs. Data are shown as mean ± SEM. Each dot represents an individual spheroid. For Brachyury, n = 42, 40, and 53, and for Sox17/Sox1 n = 46, 40, and 48 spheroids. 2 independent experiments. Kruskal-Wallis test,**p = 0.0060,****p < 0,0001; ns, non-significant.