Structural modeling of the effect of L503R in MtDMI1. Left: position of L503 in RCK1 domain, and C815 and G881 in RCK2 domain of MtDMI1. Central: position of the corresponding R814 in RCK1 and Y1126 and E1192 in the RCK2 domain of MpaDMI1. A salt bridge is predicted to connect R814 and E1192. Right: effect of L503R substitution in MtDMI1. The L503R substitution is predicted to enable the formation of salt bridges with either E813 (#1) or E609 (#2). See also Figure S4 and Tables S1 and S2.
