Microscopy from Scientific Research

Rab3GAPL reduces callose deposition at P. infestans haustoria in a catalytic-activity-dependent manner. Confocal micrographs of N. benthamiana leaf epidermal cells transiently expressing RFP:Remorin1.3 with GFP:Rab3GAPL, GFP:Rab3GAPLAIM, GFP:Rab3GAPLCM, or GFP:EV. Remorin1.3 acts as a plasma membrane and EHM marker. The leaves were infected with P. infestans spores at 1 dpi and stained with aniline blue to visualize callose at 4 dpi. Images shown are single-plane images. White arrows indicate haustoria. Scale bars represent 10 μm.

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