Rab8a overexpression rescues Rab3GAPL-mediated suppression of autophagosome numbers. Images shown are maximal projections of 27 frames with 1 μm step. Scale bars represent 10 μm. Statistical differences were analyzed by Mann-Whitney U test in R. Measurements were highly significant when p< 0.001 (***). Confocal micrographs of N. benthamiana leaf epidermal cells transiently expressing GFP:EV or GFP:Rab8a with BFP:Rab3GAPL, or BFP:EV and RFP:ATG8CL.