Marchantia polymorpha Rab3GAPL-KO mutants exhibit increased ATG8 autophagic flux. (E) Autophagic flux is estimated as the ratio of free GFP to full-size GFP:ATG8b. Western blots illustrating WT and Rab3GAPL-KO mutants in MpEF::GFP:ATG8b background after 6 h of heat stress treatment following 2 h recovery. Protein extracts were prepared using 14-day-old thalli and immunoblotted. The numbers denote the relative band intensity, quantified using ImageJ. Replicate gels are provided (Figure S3I).
