Rab3GAPL binds to different ATG8 isoforms that are transiently expressed in N. benthamiana. TagBFP:Rab3GAPL was transiently co-expressed with either GFP:ATG8 1.1, GFP:ATG8 1.2, GFP:ATG8 2.2 (CL), GFP:ATG8 3.1, GFP:ATG8 3.2, GFP:ATG8 4 (IL), or GFP:EV. Ips were obtained with rat monoclonal anti-GFP antiserum. Total protein extracts were immunoblotted. Red asterisks indicate expected band sizes. See also Figure S1.