In vitro GST pull-down assay shows Rab3GAPL-ATG8CL physical interaction is AIM dependent. Bacterial lysates containing recombinant proteins were mixed and pulled down with glutathione magnetic agarose beads. Input and bound proteins were visualized by immunoblotting with anti-GST and anti-MBP antibodies. A peptide derivative of the pathogen effector PexRD54s AIM region (AIMpPexRD54) depleted Rab3GAPL from ATG8CL complexes, whereas the mutated AIM peptide (mAIMpPexRD54) had no effect.
