HeLa-CCL2 cells transfected with Lifeact-eGFP were infected with TssB-5-mScarlet-IB. thailandensis(MOI 50). The merged images on the top show T6SS-5 (TssB-5-mScarlet-I, grayscale), host cell membranes (CellMask DeepRed, magenta), and F-actin (Lifeact-eGFP, green); individual channels shown below in gray scale. The red arrow indicates T6SS-5 assembly; the yellow arrow indicates protrusion lysis. The images were taken at 15 hpi and show an overview (on the left) and three-time points (on the right), full time-lapse inVideo S6. Scale bars represent 2 m in the magnified image and 5 m in the overview image.
