Microscopy from Scientific Research

SK-MEL-2 cells expressing dynamin-2-GFP were infected with B. thailandensisTssB-5-mScarlet-I (MOI 50). The merged images on the top show T6SS-5 (TssB-5-mScarlet-I, grayscale), host cell membranes (CellMask DeepRed, magenta), and dynamin-2-GFP (green); channels are shown separately below. The red arrows indicate T6SS-5 assemblies, and the yellow arrow indicates protrusion lysis. Imaging at 14 hpi; full time-lapse inVideo S5. Scale bar represents 2 m.

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