Example of protrusion lysis, which leads to MNGC formation. TssB-5-mScarlet-IB. thailandensis-infected HeLa cells (MOI 50). Host cell membranes (CellMask DeepRed, magenta), T6SS-5 (TssB-5-mScarlet-I, gray), and the cytoplasm (galectin-3-GFP, green). A magnified image (white box) of the bacteria that induced MNGC formation is shown underneath the merged image. Full time-lapse (from 10:40 hpi) inVideo S4. Red arrows, T6SS-5 assembly; yellow arrows, host cell membrane lysis. The scale bar represents 10 m (4 m for the magnified image).
