Example of protrusion lysis without MNGC formation. A549 cells infected with TssB-5-mScarlet-IB. thailandensis(MOI 50). The scale bar represents 5 m. The magnified image shows the escape of the bacterium from the protrusion at 0 and 1:30 min. The CellMask panel shows no MNGC formation at the 9:30 min mark. Time-lapse (from 14:40 hpi) inVideo S4. See alsoFigure S4.
