Microscopy from Scientific Research

T6SS-5 assemblies in bacteria in the host cytoplasm or with contact to the host membrane. HeLa-CCL2 cells were infected with B. thailandensis at an MOI 50 (stained with CellMask DeepRed). The top images show the merge of T6SS-5 (TssB-5-mScarlet-I, grayscale) and host cell membranes (CellMask DeepRed, magenta). The left image: cytosolic B. thailandensis with T6SS-5 sheaths. The right image: B. thailandensis in a membrane protrusion with T6SS-5 sheaths. Images below show individual channels in gray scale. Images were taken at 14 hpi. Red arrows indicate T6SS-5 assemblies. Scale bar represents 2 m.

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