HeLa-CCL2 cells expressing either galectin-3-GFP or galectin-3-mApple (mixed 1:1) were infected with B. thailandensis wild type (WT) or B. thailandensis TssB-5-mScarlet-I (MOI 50). Cells were imaged at 1718 hpi to detect the presence of cells containing both galectin-3-GFP and galectin-3-mApple, indicating MNGC formation. The left image shows a merge of the mApple and GFP channels shown individually in grayscale on the right. Scale bar represents 20 m.
