A549 lung cells infected with B. thailandensis TssB-5-mScarlet-I (gray scale) (MOI 10). Host membranes stained with CellMask DeepRed (magenta), nuclei with Hoechst 33342 (blue). Images were acquired from 5:30 to 20:00 hpi every 10 min (295 m x 295 m field of view). MNGCs are marked with a dotted red line, and bacteria outside of MNGCs are marked with yellow arrows. The scale bar represents 50 m. Time-lapse inVideo S2. See alsoFigures S1andS2.
