Workflow from Scientific Research

Cartoons illustrating the constructs and labeling conditions used in live-cell two-color single-molecule imaging. IL-5Ra (teal), IL-5 (gray and blue), and βc (red and pale red). aALFAtag and aGFP nanobodies used for labeling are depicted in gray. Effective degree of labeling (DOL) for both nanobodies = ~60%.

DOI