Steady-state fluorescence anisotropy of eIF4GGFP in cells following exposure to 30°C, 42°C, or 45°C (in HSGs) for 30 min. Mean anisotropy from 8 fields of view (FOVs) (each with 10–20 cells). Significance levels: p values * < 0.05, ** < 0.01, *** < 0.001.
