Volcano Plot from Scientific Research

Label-free quantitative mass spectrometry analyses of the sorted vesicles plotted with mCherry+ control versus mCherry+ GFP+. Curves (FDR <0.01) for HEK293 + treh/rapa + chloroquine in (D) and for Neuro2a + treh/rapa + chloroquine in (E). Inserts show vesicle sorting for mass spectrometry with mCherry+ (quadrant 1) and mCherry+ GFP+ (quadrant 2) positive signal. Receptors for Ub-substrates (pink) and TRiC subunits (purple) are highlighted; proteasome and protein quality control regulators (orange) and RNA processing factors (blue) are colored. Chloroquine was included to inhibit degradation of autophagosomal contents for mass spectrometry quantifications.

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