Image quantification of the 97Q-GFP aggregate central zone (CZ) cross-sectional area in control cells and in LC3A/B knockout cells (controln= 409, LC3A/B KOn= 551), treh/rapa-treated cells (controln= 226, +treh/rapan= 308), torin1-treated cells (controln= 264, +torin1n= 406), and cells treated with Tat-beclin1 peptide (controln= 391, +peptiden= 288). Median values are displayed, central zone intensity standardized with threshold >8,000 (Fiji),****p< 0.0001,**p< 0.01.