Dot blots with the indicated antibodies for total 150Q- and 64Q-GFP lysates from cells with 2-day muristerone A induction and removal, with a 1-day concurrent 150 mM treh/200 nM rapa treatment, or with autophagy inhibited by bafilomycin A1 (100 nM) or chloroquine (100 μM), or with Ub-activating enzyme E1 inhibited by MLN7243 (0.2 μM) for 6 or 12 h. ACTB was used as loading control.
