To monitor NMNAT1 ubiquitylationin vivo, indicated HEK293 cell lines were transiently transfected with NMNAT1-HA. Cell lysates were split in half and either mock treated or incubated with OTUB1 (±) prior to HA immunoprecipitation (IP: HA) and subsequent immunoblot analysis (top panel). Input samples confirm equal NMNAT1-HA expression (bottom panel). Vinculin serves as input control.
