To assess the half-life of endogenous NMNAT1, indicated cell lines were treated with cycloheximide (CHX) for 0–9 h, and cell lysates of each time point analyzed by immunoblot. Vinculin detection served as loading control. Quantitation of NMNAT1 immunoblot signals from (H) normalized to vinculin and relative to t = 0 values (left panel). Graph plotting relative NMNAT1 amounts, shows results by mean ± SD ofn= 5.
