Indicated cell lines were mock treated or subjected to siRNA targetingMAEA,WDR26, orYPEL5, and whole cell lysates analyzed by immunoblot (top panels). NonT, non-targeting siRNA control. Quantitation of NMNAT1 immunoblot signals normalized to vinculin and relative to NonT control values (bottom panels). Graph shows results by mean ± SD ofn= 3.pvalues were calculated using one-way ANOVA with Dunnett's multiple comparison tests.
