Immunoblot of in vitro assays testing WDR26-CTLH E3-mediated ubiquitylation of wild-type NMNAT1 and NMNAT1 variants mutating WDR26-interacting residues in the core domain (core>A) or NMNAT1 basic motif NMNAT1basic(basic>A), and NMNAT1 with basic motif deletion (delta basic).
