Coomassie-stained SDS-PAGE showing purified recombinant core-CTLH E3 (RANBP9-TWA12xS-ARMC8-RMND5A-MAEA), the supramolecular assemblies MKLN1-CTLH E3 (RANBP9-TWA12xS-ARMC8-RMND5A-MAEA-MKLN1) and WDR26-CTLH E3 (RANBP9- TWA12xS-ARMC8-RMND5A-MAEA-WDR26), and CTLH substrate NMNAT1. TWA1 is C-terminally tagged with 2xStrep.