The cleared protein lysates were incubated for 20 min at 30°C in the presence (+) or absence (-) of polyP100. Western blot with anti-nucleolin antibody revealed a considerable mobility shift of nucleolin in the samples incubated with polyP100 from both 150 and 500 mM NaCl lysates. Considering the limited extraction observed when using the lysis buffer containing 500 mM NaCl, we re-created the 500-mM salt concentration by adding extra NaCl to the 150-mM NaCl sample supernatant (500 mM*) and observed once again a dramatic nucleolin mobility shift upon polyP100 addition (+). The analysis presented is representative of three independent experiments.
