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Schematic is of lentivirus injected into the amniotic sac of E9.5 embryos of Krt14-Cre; Rosa26-LSL-Cas9 mice to specifically transduce surface epithelial skin progenitors, here with Cd80-targeting sgRNAs (EpiCd80) or use Cre negative mice as controls. Shown below are IF images and quantifications of the percentage of wound re-epithelialization (K14+) and the number of differentiated suprabasal cells (K10+) in the skins at times indicated after partial-thickness wounding. Each symbol represents a technical replicate. Results pooled from two independent experiments are shown as mean SEM (n= 3 animals for each time point in each experiment). Unpaired t test;**p< 0.01,****p< 0.0001. Scale bars, 100 m. See also Figure S2.

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