Transient DNA transfection of a caspase-3-based synpoptosis circuit including the synthetic Ras sensor module enabled selective apoptosis of RasGC cells but not WT cells. Throughout the figure, horizontal lines in the bar plots indicate the fractions established by negative and positive transient DNA transfection controls, separately for each dye and each cell line; dots represent biological replicates (distinct culture wells); bars indicate means; histograms represent three independent experiments.
