Synpoptosis circuits can selectively kill target cells by incorporating a sensor module that identifies the target cells. We used a synthetic sensor of active Ras. The sensor is a split TEVP with each half—nTEVP and cTEVP—tethered to a Ras-binding domain (RBD). In wild-type (WT) HEK cells with inactive Ras, the sensor is catalytically inactive. In cells stably expressing active Ras (RasGC cells), TEVP is reconstituted by proximity.
