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Total mRNA was extracted from the T cell blasts of three HDs, a heterozygous control (the father), P2, and P3, and EBV-B cells from three HDs, both healthy siblings, P2, and P3. Total mRNA was subjected to RT-qPCR for the assessment of FLT3LG expression with two different probes. Data are displayed as 2-Ct values normalized against the expression of endogenous GUS. Bars represent the mean values and SD.
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