Total cellular metabolite abundance (M+0 and M+1) in control and MBC KO brown adipocytes incubated with15N-BCAA at indicated time points. Data represented asZscore heatmap across 1-24 h for each metabolite, with each cell representing quantitated value for each biological replicate. n=3 per group per time point. Statistic for (A)-(E): two-way ANOVA with Sidak's multiple comparisons test.*p< 0.05;**p< 0.01;***p< 0.001;****p< 0.0001.
