Co-immunoprecipitation of Vts1-GFP. C-terminal GFP-tagged Vts1 was transformed into M. oryzae Guy11. Anti-pTEpY antiserum was used to detect double phosphorylated Pmk1. Immunoprecipitates obtained with anti-GFP antiserum and total proteins extracts were probed with appropriate antisera. Co-immunoprecipitation was repeated twice with similar results and Vts1-GFP bait protein identity was verified by LC-MS/MS.
