Illustration from Scientific Research

NMR probes indicate a conformational change of the NTD induced by the addition of 100 mM Pi ions to apo p97, reflected by peak shifts from ‘up’ (ATPS-like) to ‘down’ (ADP·Pi-like) position (the titration is shown in Extended Data Fig. 9a). This Pi concentration is above physiological intracellular levels. The same effect was observed for arsenate and sulfate ions (Extended Data Fig. 9b).

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