Yeast-two-hybrid (Y2H) assay to determine the interaction of Pmk1 with its putative direct targets. Protein interactions were tested in yeast grown on SD medium -Trp -Leu -Ade -His + alpha X-gal + Au (4DO panels). Viability of all transformed yeast cells was demonstrated by growth on SD medium -Trp -Leu (2DO panels). Yeast cells were inoculated onto media as a 10-fold dilution series. Mst12 was used as the positive control.
