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Schematic to show the infection-related development experiment in a pathogenic (Guy11) and non-pathogenic (delta pmk1)M. oryzaestrain. Spores and germinated cells were synchronized at 0, 1, 1.5, 2, 4, and 6 h. Phosphopeptides were enriched using TiO2microspheres and were subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS). Samples from 3 biological replicates were analyzed in data-dependent acquisition (DDA) mode to be quantified using label-free quantification based on precursor ions (MS1).

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