Western blot analysis of total protein extracted from in vitro germinated spores at 0, 1, 1.5, 2, and 4 h from Δpmk1, complemented withPMK1-GFPand Δpmk1, using α-pTEpY (top), α-GFP (middle), and α-actin (lower). α-pTEpY has been also reported to detect the MAPK Mps1.20Proteins were immunoblotted with appropriate antisera (listed on the right). Arrows indicate expected band sizes.
